Pirimoğlu B., Keskin C., Yüce M., Keskin M., Nuhoğlu Z., Albayrak E., ...Daha Fazla
16.Uluslararası Türk Endodonti Derneği Kongresi, Mayıs 2025
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Ödülün Kapsamı:
Bilimsel/Mesleki Çalışmalardan Alınan Ödül
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Ödül Türü:
Kongre, Konferans, Festival veya Sempozyum Kurullarınca Verilen Ödül
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Ödül Veren Ülke:
Türkiye
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Ödülü Veren Organizasyon:
16.Uluslararası Türk Endodonti Derneği Kongresi
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Araştırma Alanları:
Sağlık Bilimleri, Diş Hekimliği, Klinik Bilimler
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Ödülün Tarihi:
Mayıs 2025
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Açıklama:
<p><span style="color: rgb(19, 19, 20); font-family: "Inter Var", Inter, Roboto, Arial, sans-serif; font-size: 14px;">Periapical cyst-derived mesenchymal stem cells (pAK-MSCs) are considered potential alternative sources of mesenchymal stem cells for regenerative endodontic applications. This study aimed to compare mesenchymal stem cells derived from periapical cysts and dental pulp in terms of surface marker characterization, differentiation potential, cell viability, migratory capacity, and the secretion of odontogenic differentiation markers DMP-1 and DSPP. Material and Method: In this study, periapical cyst tissues were obtained from 10 patients who had received a preliminary diagnosis of radicular cysts and whose diagnoses were later confirmed by pathology reports. These tissues were excised through cystectomy. Additionally, dental pulp tissues were collected following the extraction of healthy impacted or semi-impacted third molars from 10 volunteers who had been indicated for extraction. The tissues were enzymatically digested using type I collagenase and dispase, and then cultured. At passage 3, the cells were compared in terms of surface antigen expression (CD13, CD26, CD44, CD73, CD90, CD105 positive; CD34, CD45 negative) using flow cytometry, clonogenic potential using the CFU-F assay, differentiation potential based on their ability to differentiate into three different cell types, viability using the MTT assay, migration capacity using the in vitro scratch test, and the levels of DMP-1 and DSPP in osteogenic medium using the ELISA test. Results: pAK-MSCs exhibited mesenchymal stem cell characteristics in terms of adherence to plastic, fibroblast-like morphology, and expression of MSC surface markers. Maximum proliferation was observed on day 5 in the pAK-MSC group (1122%) and on day 7 in the pulp group (1454%). Both groups successfully differentiated into adipocyte, chondrocyte, and osteoblast-like cells. Scratch assay results showed that pAK-MSCs achieved 90.6% wound closure at 48 hours. After osteogenic differentiation, the DMP-1 and DSPP levels in the supernatants were found to be similar in both groups (p > 0.05). Conclusion: Mesenchymal stem cells derived from periapical cysts and dental pulp exhibited similar viability, differentiation, and migration characteristics, as well as comparable levels of odontogenic marker secretion. These findings suggest that pAK-MSCs may serve as a promising alternative cell source in regenerative endodontic treatmen</span></p>