Effect of sildenafil on anxiety in the plus-maze test in mice
POLISH JOURNAL OF PHARMACOLOGY, cilt.56, sa.3, ss.353-357, 2004 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 56 Sayı: 3
- Basım Tarihi: 2004
- Dergi Adı: POLISH JOURNAL OF PHARMACOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.353-357
- Anahtar Kelimeler: sildenafil, anxiety, methylene blue, cGMP, NITRIC-OXIDE SYNTHASE, BENZODIAZEPINES
- Ondokuz Mayıs Üniversitesi Adresli: Evet
Özet
Several studies have shown a role of nitric oxide/cyclic guanosine monophosphate signaling pathway in the regulation of anxiety. The effects of the phosphodiesterase (PDE) 5 inhibitors on anxiety are not fully understood. The aim of present study was to investigate the possible role of sildenafil, an inhibitor of cyclic GMP-specific phosphodiesterase, on anxiety in the plus-maze test in mice. Sildenafil at a dose of 0.5 mg/kg had no significant effect on the behavior in the plus-maze test but at doses of 1 and 3 mg/kg induced an anxiogenic effect. The combination of sildenafil (1 mg/kg, ip) and methylene blue (1 mg/kg, ip) abolished the anxiogenic-like effect of sildenafil. The combination of sildenafil (1 mg/kg, ip) and L-arginine (50 mg/kg, ip) decreased the percentage of time spent in open arms compared to saline-treated group. Diazepam at a dose of 2 mg/kg significantly increased the percentage of time spent in open arms (p < 0.05). Sildenafil at a dose of 3 mg/kg and the combination of L-arginine (50 mg/kg, ip) and sildenafil (I mg/kg, ip) significantly decreased the locomotor activity (p < 0.05). These results suggest that a nitric oxide-cGMP pathway seems to play an important role in sildenafil-induced anxiogenic-like effect.