The role of iNOS inhibitors on lung injury induced by gastrointestinal decontamination agents aspiration
JOURNAL OF MOLECULAR HISTOLOGY, cilt.43, sa.3, ss.351-360, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 43 Sayı: 3
- Basım Tarihi: 2012
- Doi Numarası: 10.1007/s10735-012-9397-z
- Dergi Adı: JOURNAL OF MOLECULAR HISTOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.351-360
- Anahtar Kelimeler: Activated charcoal, Aspiration, Pulmonary injury, Polyethylene glycol, Surfactant protein-D, S-methylisothiourea, SURFACTANT PROTEIN-A, GLYCOL-ELECTROLYTE SOLUTION, ACUTE PULMONARY-EDEMA, ACTIVATED-CHARCOAL, COMPLICATIONS, BRONCHIOLITIS, PNEUMONIA, MODEL, RISK
- Ondokuz Mayıs Üniversitesi Adresli: Evet
Özet
Aspiration is a devastating complication during decontamination procedure in poisoning patients. We have investigated whether S-methylisothiourea protects different pulmonary aspiration gastrointestinal decontamination agent-induced lung injury in rats. Forty-two male Sprague-Dawley rats were assigned to one of six groups (n = 7): normal saline, activated charcoal, polyethylene glycol, normal saline + S-methylisothiourea treated activated charcoal + S-methylisothiourea treated and polyethylene glycol + S-methylisothiourea treated. Normal saline, activated aharcoal and polyethylene glycol were instilled into the lungs. The rats received S-methylisothiourea i.p twice daily for 7 days. Serum surfactant protein D, oxidative stress products and inducible nitric oxide synthase expression in the lung were investigated. The aspiration of activated charcoal significantly increased all histopathological scores (P < 0.01). Only peribronchial inflammatory cell infiltration, alveolar edema, and alveolar histiocytes were increased in the polyethylene glycol groups as compared to the normal saline group (P < 0.05). Pulmonary aspiration increased serum malondialdehyde (P < 0.001), and surfactant protein D (P < 0.05) levels and decreased serum superoxide dismutase levels (P < 0.05). S-methylisothiourea treatment decreased all histopathological scores in the activated charcoal treated S-methylisothiourea group (P < 0.01) and only decreased alveolar edema and alveolar histiocytes in the polyethylene glycol-treated S-methylisothiourea group (P < 0.05). S-methylisothiourea treatment reduced elevated oxidative factors, inducible nitric oxide synthase activity and serum surfactant protein D levels. Our findings showed that S-methylisothiourea may be a protective drug against Activated Charcoal and Polyethylene Glycol-induced lung injury.