Background: Ovarian cysts are commonly observed pathologies, which interfere with normal cyclic activity and adversely affect fertility in cows. Beta-carotene is effective in the reduction of reproductive problems by inducing the natural defence mechanisms of the body. There are several methods that can be used for the treatment of ovarian cysts. The separate and combined use of GnRH and PGF2 alpha commonly uses in the treatment of ovarian cysts. Therefore, in the presented study the effects of Beta-carotene (beta c) addition for the treatment of ovarian cysts either with GnRH solely or GnRH and PGF2 alpha in combination on the fertility parameters of dairy cows were investigated.