Resveratrol Ameliorates PTZ-Induced Seizure Activity and Diaphragm Muscle Dysfunction by Regulating Calcium Handling and Apoptotic Pathways in Rats
MOLECULAR NEUROBIOLOGY, cilt.63, sa.1, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 63 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s12035-026-06054-w
- Dergi Adı: MOLECULAR NEUROBIOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, EMBASE, MEDLINE, Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest), Pharma Collection (ProQuest)
- Ondokuz Mayıs Üniversitesi Adresli: Evet
Özet
This study aimed to investigate the chronic effects of epilepsy, a common health problem, on the diaphragm muscle and the therapeutic potential of resveratrol against these effects. Thirty-two adult male Wistar rats were divided into four groups (n = 8): Sham, resveratrol (Res, 5 mg/kg/day), pentylenetetrazol (PTZ), and PTZ + Res. Seizure activity and frequency in the epileptic groups were monitored via electrocorticography (ECoG) recordings. Diaphragmatic mechanical activities were evaluated through basal contractility and frequency-dependent fatigue parameters. Molecular analyses focused on calcium homeostasis, including tissue calcium (Ca2+) levels and sarcoplasmic/endoplasmic reticulum Ca2+-transporting ATPase 2 (Atp2a2) mRNA expression, as well as apoptotic markers (Casp-3, Casp-9, Bax, and Bcl-2). Our results indicated a significant suppression of diaphragmatic contractile parameters induced by chronic seizures, as verified by ECoG. This suppression was a consequence of Atp2a2 inhibition, Ca2+ overload, and increased apoptotic activity. On the other hand, resveratrol treatment exhibited a therapeutic effect toward restoring basal mechanical activities of the diaphragm and gaining resistance against fatigue. This effect was mediated through the reorganization of Ca2+ homeostasis and the inhibition of increased apoptotic activity. In conclusion, resveratrol demonstrated promising results in improving diaphragmatic functions in individuals with epilepsy, thanks to its role in suppressing epileptic activity, regulating Ca2+ and Atp2a2 management, and its antiapoptotic effects.