Tez Türü: Doktora
Tezin Yürütüldüğü Kurum: Ondokuz Mayıs Üniversitesi, Lisansüstü Eğitim Enstitüsü, Nanobilim ve Nanoteknoloji Anabilim Dalı, Türkiye
Tez Danışmanı: Sinem Çevik
Tezin Onay Tarihi: 2023
Tezin Dili: İngilizce
Özet:
The present study was designed to applicate the coating of metallic biomaterials used in dentistry with polymer materials containing plant extracts. The electrospray deposition (ESD) technique, which is a low-cost and practical method, was used to form micrometer-scale coatings produced with nanoparticles produced by green synthesis, plant extracts, and Polyvinyl Alcohol (PVA). The properties of nanoparticles and polymer composite coating materials produced using Hemp seeds, St. John's Wort, Rosemary and Cherry Laurel Leaf extracts were investigated and compared with different concentrations and plant groups. The effects of fabrication technique and initial composition on the morphology, thermal, mechanical, and chemical behavior of nanocomposite coatings are investigated and discussed. Scanning Electron Microscope (SEM) analysis showed that green synthesized nanosilver was produced with an average size of 34 nm and homogeneous size and distribution. Dynamic mechanical analyses showed that the samples produced with 0.3% (D) and 0.5% (E) nanosilver modifications were the ones with the highest thermal and mechanical strength after coating. Antibacterial tests revealed that all nanosilver-added coatings reduced bacterial growth up to 100 times, and the sample with the highest antibacterial effect was the 0.5% nanosilver-modified E sample. The scratch test applied to determine the coating's mechanical properties showed that the hardest samples among the coating surfaces were 0.1% (C), 0.3% (D), and 0.5% (E). In addition, it was determined that the mechanical strength of the coatings increased at least two times with the modification of plant extracts to PVA compared to the pure coatings. It was determined that the chemical and mechanical stability of nanosilvermodified C, D, and E samples in water at 37 °C were higher than the other samples. Toxicity tests confirmed that the green synthesized nanosilver did not show toxic effects on HaCaT live cells and A549 lung cancer cells with concentrations at 62 µg/mL and below in coatings. The present study has offered a framework for the exploration of plant extracts and nano-silver-modified polymer nanocomposite coating applications in the health field. This study provided a framework for investigating plant extracts and nano-silvermodified polymer nanocomposite coating applications in the healthcare field. In light of the empirical findings obtained, this study offers a new understanding of the antibacterial and biocompatible production of metallic biomaterials using plant extracts. In addition, it is thought that these biocomposite materials will have a wide range of uses in scientific and biomedical environments thanks to their low raw material and production costs, easy production, human health-friendly and environmentally friendly production methods, high thermomechanical strength, and low toxic effect compared to their equivalents