Effect of Cumulative Zinc Doses on Papillary Muscle Contractions and the Zinc Finger Protein ZEB1

Akgun-Unal N., Ustun A., Bayirli S., Unal O., Mogulkoc R., Baltaci A. K.

BIOLOGICAL TRACE ELEMENT RESEARCH, 2025 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1007/s12011-025-04550-z
  • Dergi Adı: BIOLOGICAL TRACE ELEMENT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, Pollution Abstracts, Veterinary Science Database
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

In this study, we aimed to investigate the effects of cumulative doses of Zn2+ (by exposing samples to 1 mu M, 10 mu M, and 100 mu M ZnCl2) on myocardial papillary muscle contractions isolated from rat hearts in vitro and the roles of the zinc finger protein ZEB1 in this effect. In these preparations, 100 mu M ZnCl2 application in different protocols caused a decrease in contraction force and an increase in contraction time in both frequency-dependent parameters and pre-expected stimuli when compared to the control group. Our study data show that Ca2+ homeostasis is closely related to increasing Zn2+ doses (especially at 100 mu M ZnCl2 dose). Secondly, the levels of ZEB1, a zinc finger protein, were also significantly lower in the 100 mu M ZnCl2 group compared to the other groups, which seems to be related to the increase in Ca2+ that triggers ROS production at high doses of Zn2+. The data of our study, which we conducted to understand the Zn2+ concentrations in the heart and to reveal new mechanisms that play a role in the regulation of Ca2+ dynamics in heart tissue and is the first research in the literature on this subject, show that in vitro zinc application may have a dose-dependent effect on myocardial papillary muscle contractions.