Subclinical mastitis (SCM) is a serious disease of farm animals. There are many studies on SCM that aim to makethe diagnosis practical, economical and reliable. It was the aim of the present study comparison of epidermalgrowth factors (EGF) ratios in animals with and without subclinical mastitis as an indictor of SCM in Anatolianwater buffaloes. A study was carried out in a total of on 35 milk samples from 20 healthy and 15 mastiticmammary quarters of clinical healthy and nonpregnant buffaloes. Samples were examined using Californiamastitis test (CMT) as cow side test to detect of SCM before being transferred to the laboratory. Microbiologicalculture was also performed to determine bacterial contamination. Level of EGF and somatic cell count (SCC) wasdetermined in all samples. As a result of the comparison of the EGF concentration between the SCM (+) andSCM (-) groups, the SCM (+) group was found to be higher than the SCM (-) group. EGF tests may be a usefultool in determining subclinical mastitis.