Investigation of the nephroprotective effect of dexmedetomidine on colistin-induced nephrotoxicity in rats
BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY, vol.123, no.8, pp.579-584, 2022 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 123 Issue: 8
- Publication Date: 2022
- Doi Number: 10.4149/bll_2022_094
- Journal Name: BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE
- Page Numbers: pp.579-584
- Keywords: rat, colistin, nephrotoxicity, dexmedetomidine, ISCHEMIA-REPERFUSION INJURY, ACUTE KIDNEY INJURY, RENAL-FUNCTION, INFUSION
- Open Archive Collection: AVESIS Open Access Collection
- Ondokuz Mayıs University Affiliated: No
Abstract
BACKGROUND: There are very few studies in the literature focusing on whether dexmedetomidine exerts a protective effect on colistin nephrotoxicity. Our study aims to investigate the nephroprotective effect of dexmedetomidine in an experimental model of nephrotoxicity in rats. METHODS: The control group was administered saline (SF) intraperitoneally twice a day. The colistin group received an intraperitoneal (ip) injection of 10 mg/kg of colistin twice a day. The DX10 group received 10 mg/ kg of colistin 20 minutes after the intraperitoneal injection of 10 mcg/kg of dexmedetomidine. The DX20 group received 10 mg/kg of colistin 20 minutes after the intraperitoneal injection of 20 mcg/kg of dexmedetomidine. Applications were continued for 7 days, twice a day. All rats were sacrificed on the 8th day after blood and kidney tissue samples were taken. BUN, Creatine, KIM-1 and Endothelin-1 were studied in blood samples. RESULTS: There was a significant difference in the median values of Urea, BUN and Creatine between the groups (p<0.001, p<0.001, p<0.001, respectively). There was a significant difference in the median values of KIM-1 and Endothelin-1 between the groups (p=0.009, p=0.001, respectively). A significant difference was observed between the histopathological scores of the groups (p<0.001). CONCLUSION: Dexmedetomidine significantly decreased the elevated levels of BUN, Creatinine, KIM-1, and Endothelin-1 induced by colistin. Dexmedetomidine, at both doses, histopathologically prevented apoptosis and reduced the number of necrotic cells in the kidneys. Dexmedetomidine provides renoprotective effects, therefore it is a valuable sedation agent for clinicians working in intensive care units (Tab. 2, Fig. 4, Ref. 19). Text in PDF www.elis.sk