Akademik Veri Yönetim Sistemi
Kocatepe Veterinary Journal, cilt.18, sa.2, ss.164-170, 2025 (TRDizin)
This study aimed to evaluate the effects of extended-spectrum beta-lactamase (ESBL) producing and non-producing Escherichia coli concentrations on bull semen motility parameters and spermatozoa viability. A total of 50 frozen semen straws from the same Simmental bull were used. All semen straws were thawed in a water bath at 37 °C and divided into 5 groups of 10 semen samples. Group 1 (G1) (n=10) was the uncontaminated control group. Group 2 (G2) (n=10) was contaminated with 100.000 cfu/ml and Group 3 (G3) (n=10) was contaminated with E. coli ATCC 25922 at a concentration of 1.000.000 cfu/ml. Group 4 (G4) (n=10) was contaminated with 100.000 cfu/ml and Group 5 (G5) (n=10) was contaminated with ESBL producing E. coli BAA-196 at a concentration of 1.000.000 cfu/ml. In the study, progressive motility, motility and percentages of dead-live spermatozoa in semen samples of these groups were analyzed over time. Significant decreases in spermatozoa motility and viability were observed and the most significant effects were seen in groups (G4 and G5) contaminated with high concentrations of ESBL-producing E. coli (p<0.05). It was determined that the effect of bacterial contamination on spermatological parameters was dose-dependent, with higher concentrations causing more rapid and severe deterioration in semen quality. These findings highlight the role of bacterial contamination, especially with resistant strains, in reducing semen quality and draw attention to the importance of microbial contamination in artificial insemination practices. Further research is needed to explore alternative methods to control contamination in reproductive technologies and combat antibiotic resistance.