Environmental concentrations of antifouling biocides affect cell proliferation, possibly by a synergic interaction

Tokur O., Aksoy A.

JOURNAL OF SEA RESEARCH, vol.191, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 191
  • Publication Date: 2023
  • Doi Number: 10.1016/j.seares.2022.102330
  • Journal Name: JOURNAL OF SEA RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, CAB Abstracts, Communication Abstracts, Geobase, INSPEC, Metadex, Veterinary Science Database, Directory of Open Access Journals, Civil Engineering Abstracts
  • Keywords: Cytotoxicity, DCOIT, Dichlofluanid, EPC, Zineb, 4,5-DICHLORO-2-N-OCTYL-4-ISOTHIAZOLIN-3-ONE DCOIT, BOOSTER BIOCIDES, MARINE MACROALGA, IN-VITRO, TOXICITY, ZINEB, DICHLOFLUANID, PESTICIDES, GROWTH, TOLYLFLUANID
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

Antifouling biocide accumulation is a significant problem in aquatic environments. Most research focuses on biocides' individual toxicity; however, because biocides are mixed in water, toxic interactions may intensify their harmful effects. Therefore, this study investigated the cellular (EPC cell line) toxicity of three common anti-fouling biocides, individually and in combination. 24-hour and 96-hour IC50 levels of the biocides were 1.11-1.03 mg L-1 (DCOIT), 7.31-11.94 mg L-1 (dichlofluanid), and 54.98-78.06 mg L-1 (zineb). DCOIT + dichlofluanid combination was synergic, whereas other combinations were antagonists. Environmentally rele-vant levels of combinations were also tested, and only DCOIT + dichlofluanid combination affected cell pro-liferation rates at 96 h (p = 0.035) compared to the untreated control. We concluded that biocides might interact synergically, thus enhancing their adverse impacts, and constant exposure to the biocides at environmentally relevant concentrations may be deleterious to aquatic species.