Phytochemical Analysis of Pyracantha angustifolia: Synthesis of Silver Nanoparticles, Characterization, Antimicrobial Activity, and In Silico Studies

Başar, Yunus; Yiğit, Aybek; Dönmez, Mesude; Demirtaş, İbrahim; Alma, Mehmet

doi:10.18016/ksutarimdoga.vi.1593890

Phytochemical Analysis of Pyracantha angustifolia: Synthesis of Silver Nanoparticles, Characterization, Antimicrobial Activity, and In Silico Studies

Başar Y., Yiğit A., Dönmez M. F., Demirtaş İ., Alma M. H.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, cilt.29, sa.1, ss.1-14, 2025 (ESCI, TRDizin)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 29 Sayı: 1
Basım Tarihi: 2025
Doi Numarası: 10.18016/ksutarimdoga.vi.1593890
Dergi Adı: KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE
Derginin Tarandığı İndeksler: Agricultural & Environmental Science Database, Emerging Sources Citation Index (ESCI), CAB Abstracts, TR DİZİN (ULAKBİM)
Sayfa Sayıları: ss.1-14
Ondokuz Mayıs Üniversitesi Adresli: Hayır

Özet

Nanotechnology is rapidly advancing across various industries, highlighting the need for environmentally friendly nanoparticle (NP) synthesis methods. In this study, silver nanoparticles (AgNPs) were synthesized using Pyracantha angustifolia (PA), also known as firethorn, a plant with applications in food and pharmaceuticals. The AgNPs were characterized via UV-VIS, FT-IR, FE-SEM, FESEM-EDX, and their antibacterial activity was tested against Erwinia caratovora and Clavibacter michiganensis. Phytochemical analysis of PA aqueous extract identified hesperidin (1240.3564 ng/ml) and palmitic acid methyl ester (30.79%) as major components using LC-ESI-MS/MS and GC-MS/MS, respectively. AgNPs of PA showed high antibacterial activity against the pathogens E. caratovora and C. michiganensis. In silico studies confirmed hespiridin’s interaction with DNA gyrase (MolDock score; -123.43, binding energies; -7.00 kcal/mol and ΔG binding values; 15.051 kj/mol), supporting its potential as an antibacterial agent.