A Saccharopolyspora strain, designated CR3506(T), isolated from a soil sample collected from Sungurlu, Corum, Turkey, was examinated using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence analysis showed that the strain is closely related to the type strains of Saccharopolyspora spinosa NRRL 18395(T) (99.1%), Saccharopolyspora phatthalungensis NRRL B-24798(T) (98.4%) and Saccharopolyspora shandongensis 88(T) (98.1%); low levels of DNA-DNA relatedness were found between the isolate and S. spinosa and S. phatthalungensis (< 50%). Strain CR3506(T) was found to have chemotaxonomic and phylogenetic properties consistent with its classification in the genus Saccharopolyspora. The strain contained meso-diaminopimelic acid as the diagnostic diamino acid. Whole-cell hydrolysates contained arabinose and galactose. The polar lipids were identified as phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones (> 10%) were MK-9(H-4) and MK-8(H-4). Major fatty acids were (> 10%) iso-C-16:0, C(15:0)3OH, C-18:0 and iso-C-15:0. Further, the morphological, physiological and biochemical characteristics of strain CR3506(T) are distinct from S. spinosa and other species of the genus Saccharopolyspora with which this strain has high 16S rRNA gene sequence similarity (98.0-98.5%). Strain CR3506(T) has antimicrobial activity against Bacillus subtilis NRRL B-209, Citrobacter freundi NRRL B-2643 and Staphylococcus aureus ATCC 29213. Consequently, it is proposed that strain CR3506(T) represents a novel Saccharopolyspora species for which the name Saccharopolyspora hattusasensis sp. nov. is proposed. The type strain is CR3506(T) (=KCTC 29104(T) = DSM 45715(T)).