The aim of this study is to examine the direct, indirect and total effects of nutrient content properties on metabolizable energy contents of natural pasture feeds using path analysis. For this aim, the relationship between metabolizable energy and eight feed nutrient characteristics such as acid detergent lignin (ADL), acid detergent fiber (ADF), neutral detergent fiber (NDF), crude fiber, crude protein, ether extract, ash, and the nitrogen-free extract (NFE) was used for 48 grassland plants samples obtained from natural pastures of Ankara. According to the results of path analysis, the most significant effects on metabolizable energy were reported in crude fiber, acid detergent lignin, crude protein, ash, and nitrogen-free extract. In addition, both acid detergent lignin and neutral detergent fiber had a negative and significant impact on metabolizable energy. As a result, acid detergent lignin and neutral detergent fiber can be presented as prediction criteria to predict metabolizable energy.