Development of qualitative and quantitative ELISA models for bovine brucellosis diagnosis Sıgǧir brusellozis tanısı için kalitatif ve kantitatif ELISA modellerinin geliştirilmesi

Genç O., Büyüktanir Ö., Yurdusev N.

Kafkas Universitesi Veteriner Fakultesi Dergisi, vol.16, no.2, pp.287-291, 2010 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 2
  • Publication Date: 2010
  • Journal Name: Kafkas Universitesi Veteriner Fakultesi Dergisi
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.287-291
  • Keywords: Bovine brucellosis, ELISA, IgG antibody, LPS, Quantitative ELISA
  • Ondokuz Mayıs University Affiliated: Yes


In this study, a qualitative and a quantitative ELISA models (iELISA and qELISA, respectively) have been developed for screening and quantifying IgG isotype anti-Brucella abortus LPS antibody. For developing high sensitive and specific ELISA models, the nature and concentration of blocking-diluting reagents have been found very critical since milk components such as casein and particularly β-lactoglobulin, but not α-lactalbumin, strongly inhibited the anti-LPS antibody detection by an unknown mechanism. The iELISA as well as qELISA models have been developed and validated by OIE reference sera and well-known field sera evaluated with RBT, CRT and cELISA. The results demonstrated that both ELISA models, iELISA in large-scale screening and qELISA in standard determination of anti-LPS antibody concentration, developed in this study can be included as valuable and effective immunodiagnostic tools in brucellosis monitoring-eradication and vaccination surveillance programs in endemic countries.