In Vitro Cytotoxic Evaluation of a Silver(I) Complex Including Non-Steroidal Anti-Inflammatory Drug Niflumic Acid and 3-Picoline on Human-Derived Cancer Cell Lines

Çağlar S., Altay A., Çağlar B., Kağan Yeniçeri E. K., Harurluoğlu B.

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.15, no.3, pp.736-748, 2022 (Peer-Reviewed Journal)

Abstract

Here, a novel silver(I) complex including the non-steroidal anti-inflammatory drug niflumic acid and 3-picoline was synthesized and characterized by FT-IR, 1H NMR, elemental and thermal analysis techniques. These techniques demonstrated that the formula of the synthesized complex is [Ag(nif)(3-pic)]. The cytotoxic ability of the complex, ligand (niflumic acid) and silver ions alone were tested against human breast adenocarcinoma (MDA-MB-453), lung adenocarcinoma (A-549), colorectal adenocarcinoma (HT-29), and mouse fibroblast (3T3-L1) cell lines. The XTT results indicated that although AgNO3 and niflumic acid alone exhibited modest cytotoxicity on the cancer and healthy cell lines, the complex indicated strong cytotoxic activity on the cancer cells in dose-dependent manner. The strongest cytotoxicity and the highest selectivity by the complex were determined on HT-29 cells. These findings provide fundamental outputs for the evaluation of the novel silver(I) complex in advanced anticancer studies.