HPLC analysis, anti-oxidant activity of Genista ferox and its antiproliferative effect in HeLa cell line

Bencherchar I., Demirtas İ., Altun M., Gül F., Sarri D., Benayache F., ...More

Bangladesh Journal of Pharmacology, vol.12, no.3, pp.260-267, 2017 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 3
  • Publication Date: 2017
  • Doi Number: 10.3329/bjp.v12i3.32310
  • Journal Name: Bangladesh Journal of Pharmacology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.260-267
  • Ondokuz Mayıs University Affiliated: No


The prevention and treatment of the cancer using plants have attracted increasing interest. The present study was aimed to determine the phenolic compounds of Genista ferox using HPLC-TOF/MS and the anti-oxidant activity associated with anti-cancer activity against human cervical adenocarcinoma (HeLa) cell line. Total anti-oxidant capacities of different extracts of G. ferox were assessed by DPPH assay, and their total phenolic and flavonoids contents measured by Folin-Ciocalteu and aluminum trichloride assays. The amounts of total phenolic (105.2 ± 0.6 - 308.5 ± 5.7 mg/g) of extract measured as gallic acid equivalent and flavonoids (8.1 ± 0.1 - 124.0 ± 0.7 mg/g) of extract measured as quercetin equivalent varied from chloroform to n-butanol extract of the two parts of the plant (leaf and stem). The ethyl acetate extract of G. ferox exhibited the most powerful effect on the DPPH scavenging activity with 94% from the leaf and 93% from the stem, while the chloroform extract from the leaf exhibited the most effective anti-proliferative activity against HeLa cell lines.