Akademik Veri Yönetim Sistemi
BIOMEDICINES, cilt.13, sa.11, ss.1-23, 2025 (SCI-Expanded, Scopus)
Background/Objectives: Carnosine and exercise independently improve metabolic
health, yet their combined effects on myokines and microbiota-derived
metabolites remain underexplored. This study evaluated the synergistic impact
of carnosine supplementation and exercise intensity on microbiota-derived
metabolites, as well as skeletal muscle and myocardial expression of irisin and
myonectin, focusing on lipid and glycemic regulation. Methods: A randomized post-test control study was conducted
using 49 male Sprague Dawley rats (9 weeks old;
250.39±1.85g), divided into 7 groups: control (C), sham (S), moderate-intensity
continuous training (MICT), high-intensity continuous training (HICT),
carnosine (CA), MICT with carnosine (MICTCA), and HICT with
carnosine (HICTCA). Interventions included treadmill-based moderate
or high-intensity training, carnosine supplementation (100mg/kg/day) for 5
weeks. Blood samples were collected post-decapitation; plasma was analyzed for
lipid profile, glycemic parameters, and microbiota-derived metabolites using
enzymatic and ELISA methods. Irisin and myonectin levels were assessed in
plasma and myocardial and skeletal muscle tissues via ELISA and
immunohistochemistry. Results: The HICTCA
group showed the lowest body weight, highest HDL-C, and lowest LDL-C, TC, TG,
and atherogenic index. Irisin and myonectin levels in skeletal muscle and
myocardium were also highest in HICTCA. The trimethylamine N-oxide
(TMAO) was lowest and S-equol highest in HICTCA, whereas indoxyl
sulfate (IS) peaked in HICT and was lowest in the C group. Principal component
analysis revealed strong positive associations between HICTCA and
cardiometabolic biomarkers. Conclusions: High-intensity
training combined with carnosine may reduce weight gain, improve lipid and
glycemic profiles, and enhance myokines and microbiota-derived metabolites.