The banana (Musa sp.) plant grows in tropical and subtropicalregions. The fruit of the banana is eaten and the peel of thebanana is discarded. In this study, chemical analyzes (HPLCTOF/MS ve GC-MS) and biochemical activities (antioxidantand antimicrobial) of ethyl acetate (Ea) andmethanol/chloroform (Me/Ch) extracts prepared from bananapeel (Bbp) grown in Brazil and banana peel (Tbp) grown inTurkey were investigated. Cetene, 4-hydroxybenzoic, palmitic,linoleic, stearic, gentisic and syringic acid are the mostabundant compounds in the extracts. The total antioxidantactivity of Tbp-Ea (A0.5: 36.84 μg/mL), inhibition of lipidperoxidation of Bbp-Ea (IC50: 3.22 μg/mL), reducing power ofTbp-Me/Ch (A0.5: 4.96 μg/mL), DPPH• scavenging activity ofBbp-Ea (IC50: 8.54 μg/mL), metal chelating activity of Tbp-Ea(IC50: 16.54 μg/mL) and H2O2 scavenging activity of TbpMe/Ch (IC50: 0.36 μg/mL) were found the most effective. Itwas exhibited that extracts were effective against gramnegative and gram-positive bacteria. In conclusion, thephytochemical and biochemical results of the TbP and Bbpextracts will contribute further pharmacological, biochemicaland pharmacological investigations.