Akademik Veri Yönetim Sistemi
“2. Uluslararası Tarım, Çevre ve Sağlık Kongresi , Aydın, Türkiye, 18 - 19 Ekim 2019, ss.677-688, (Tam Metin Bildiri)
Actinobacterial strains isolated from arid soil samples were screened for their ability for extracellular synthesis of
silver nanoparticles (AgNPs). Thirteen strains showed evidence for extracellular synthesis of AgNPs, among
which strains SH121, MTSM24 and SHAY3-32 showed high potency. Highest yield was obtained from SH121-
mediated extracellular AgNPs synthesis after effect of some parameters (reaction temperature, pH of reaction
mixture and initial silver nitrate concentration) on synthesis was determined. It was found that 16S rRNA gene
sequence of isolate SH121 was 99.3% similar to that of Micromonospora chokoriensis DSM 45160T. AgNPs
synthesized by SH121 under optimum reaction conditions (40 °C, pH 7.0, 2 mM silver nitrate concentration)
exhibited an absorption maximum at 402 nm, caused by their Surface Plasmon Resonance (SPR) vibration.
Scanning Electron Microscopy (SEM) analysis showed that the synthesized AgNPs were spherical in shape.
Dynamic light scattering (DLS) analysis also showed that they were well dispersed with a size ranging from 6 to
40 nm. Furthermore, AgNPs showed antibacterial effect against Escherichia coli, Staphylococcus aureus, Bacillus
subtilis, Enterococcus hirae, and Legionella pneumophila subsp. pneumophiia. Besides, AgNPs did not show
significant cytotoxicity when the nanoparticles were exposed to fibroblast cell line (BJ5TA) at low concentrations
(1 and 10 μg/mL).