Research Information System
Pediatric Nephrology, vol.13, no.4, pp.326-332, 1999 (SCI-Expanded)
Erythrocytes are target cells for peroxidative damage. Abnormal susceptibility of erythrocyte lipids to peroxidation is believed to reflect a similar abnormality in other organs and tissues. The changes in erythrocyte lipid peroxidation [measured by malonyldialdehyde (MDA) concentration] and erythrocyte membrane cholesterol (EMC) and their correlation with plasma lipid changes were studied in 36 children with steroid-responsive minimal change nephrotic syndrome (MCNS) (16 in relapse, 20 in remission) and 20 matched healthy controls. Erythrocyte MDA levels were significantly higher in relapse [126.3 ± 40.6 nmol/g hemoglobin (Hb)] compared with remission (101.2 ± 21.3 nmol/g Hb, P < 0.02) and in controls (95.4 ± 20.4 nmol/g Hb, P < 0.001). Plasma MDA levels in relapse were also higher than in remission (4.26 ± 1.19 nmol/ml vs. 3.16 ± 1.18 nmol/ml, P < 0.01), and in controls (2.49 ± 0.86 nmol/ml, P < 0.001). The EMC content changed significantly during remission (1.22 ± 0.15 mg/1010 cells in relapse, 1.09 ± 0.19 mg/1010 cells in remission, P < 0.04). These results show an increased sensitivity of red cells to lipid peroxidation in patients with steroid-sensitive nephrotic syndrome without the development of renal failure and anemia. Lipid peroxidation of plasma lipids and erythrocyte membrane may be a primary phenomenon, but this should be confirmed by investigation of peroxidation of renal lipids.