The aim of the present study was to determine the availability of blood and milk lactoferrin concentrations
in the diagnosis of subclinical mastitis in lactating Karayaka ewes. The milk samples obtained from 43 mid-lactating
Karayaka ewes, aged between 2 and 3, were examined by California Mastitis Test (CMT) and somatic cell count (SCC)
methods. Animals (n=23) had positive CMT score and SCC between 300.000-1.000.000 cells/ml were defined as subclinical
mastitis group, whereas ewes with CMT negative and SCC between 100.000-200.000 cells/ml were described
as control (healthy) group. The serum were obtained from blood and milk samples of ewes and ELISA was performed to
detect blood and milk lactoferrin concentrations. The mean blood lactoferrin concentrations in subclinical mastitis group
and control group were 0.90±0.08 μg/ml and 0.85±0.08 μg/ml, respectively, however the difference was not significant
(p>0.05). The mean milk lactoferrin concentrations in ewes with subclinical mastitis and healthy ewes was 23.98±3.45
μg/ml and 16.84±1.29 μg/ml, respectively (p
Bu çalışmada, laktasyon periyodundaki subklinik mastitisli ve sağlıklı Karayaka ırkı koyunlarda süt ve kan serumlarında
laktoferrin konsantrasyonlarının belirlenmesi amaçlandı. Araştırmada, ortalama 2-3 yaşlarında ve laktasyonlarının
orta döneminde bulunan Karayaka ırkı koyunlar, Kaliforniya mastitis test ve sütte somatik hücre sayımı
yöntemleri ile subklinik mastitis yönünden incelendi. Sütleri Kaliforniya mastitis test pozitif ve somatik hücre sayımı
300.000-1.000.000 olan 23 adet koyun subklinik mastitis grubunu, Kaliforniya mastitis test negatif ve somatik hücre
sayımı 0.05). Süt serumu laktoferrin
konsantrasyonları subklinik mastitisli ve kontrol gruplarında sırasıyla 23.98±3.45 μg/ml ve 16.84±1.29 μg/ml olarak
bulundu. Subklinik mastitisli grubun süt serumu laktoferrin düzeylerinin kontrol grubununkine göre istatistiksel olarak
yüksek olduğu belirlendi (p
