Quantification of applied dose in irradiated citrus fruits by DNA Comet Assay together with image analysis

Cetinkaya N., Ercin D., Ozvatan S., Erel Y.

FOOD CHEMISTRY, vol.192, pp.370-373, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 192
  • Publication Date: 2016
  • Doi Number: 10.1016/j.foodchem.2015.07.027
  • Journal Name: FOOD CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.370-373
  • Keywords: Irradiation, DNA Comet Assay, Quarantine control method, Citrus fruits, Image analysis, FOOD
  • Ondokuz Mayıs University Affiliated: Yes


The experiments were conducted for quantification of applied dose for quarantine control in irradiated citrus fruits. Citrus fruits exposed to doses of 0.1 to 1.5 kGy and analyzed by DNA Comet Assay. Observed comets were evaluated by image analysis. The tail length, tail moment and tail DNA% of comets were used for the interpretation of comets. Irradiated citrus fruits showed the separated tails from the head of the comet by increasing applied doses from 0.1 to 1.5 kGy. The mean tail length and mean tail moment% levels of irradiated citrus fruits at all doses are significantly different (p < 0.01) from control even for the lowest dose at 0.1 kGy. Thus, DNA Comet Assay may be a practical quarantine control method for irradiated citrus fruits since it has been possible to estimate the applied low doses as small as 0.1 kGy when it is combined with image analysis. (C) 2015 Elsevier Ltd. All rights reserved.