Akademik Veri Yönetim Sistemi
13th European Congress of Andrology, Stockholm, İsveç, 4 - 06 Eylül 2024, ss.118
Objective: The study aimed to evaluate the microbial content and diversity in semen samples of obese men, determine the differences between infertile and fertile groups, and investigate the effect of seminal microbiota on semen parameters, sperm DNA fragmentation (SDF),sperm chromatin condensation, and total antioxidant capacity (TAC).
Material and Method: Thirteen obese infertile men with a BMI above30 kg/m2 and five obese fertile men with children younger than 2 years of age as the control group were included in the study. For microbiome analysis, the V3 and V4 regions of the 16S ribosomal RNA gene were sequenced using the amplicon sequencing method. SDF and TAC were analyzed by the TUNEL and the ELISA, respectively. Sperm chromatin condensation was evaluated by the aniline blue staining method.
Results: The most abundant bacteria in both groups were seen tobe belonging to the phylum Bacillota, Pseudomonadota, Actinomycetota, and Bacteroidota. The common bacteria at the genus level were Pseudescherichia, Staphylococcus, Paenibacillus, Streptococcus, Klebsiella,and Moraxella, which had similar distributions in both groups. A negative correlation was observed between the percentage of histone-rich sperm and motility (p < 0.0001), sperm concentration (p = 0.0001), and total sperm count (p = 0.001). It was found that Brevibacterium, Paenibacillus, Alistipes, Lactiplantibacillus, Rhizobacter, Sphingomonas, and Venlonella genera were correlated with SDF; Pantoea, Devosia, Bacteroides,and Acidovorax genera were associated with TAC; Fusobacterium genera was correlated with the histone-rich sperm, and Corynebacterium, Hydrogenophaga, and Paenalcagenes genera were associated with BMI.
Conclusion: Bacterial species in semen may play a role in male infertility by affecting semen quality, sperm DNA fragmentation, or total antioxidant capacity.