In this study, 15 Yersinia ruckeri isolates that had been isolated from rainbow trout farms and 2 reference strains (serotype 1 and serotype 2) were examined in terms of phenotypic, serotypic and genotypic characteristics. Conventional microbiological and API 20 E tests were used to determine phenotypic characteristics of Y. ruckeri isolates and it was determined that the Y. ruckeri showed significantly homogenous profile by microagglutination test performed by using serotype 1 and serotype 2 immunsera, and 11 of 15 isolates were serotyped as serogroup 1 while the rest 4 were serotyped as serogroup 2. In Random Amplified Polymorphic DNA (RAPD) analysis, Y. ruckeri isolates were genotyped within 2 separate clusters according to 70% similarity coefficient index and it was detected that first cluster includes 2 genotypes (YR1 and YR2) and second cluster includes 3 genotypes (YR3, YR4 and YR5). Furthermore, antibiotic resistance profiles of Y. ruckeri strains were determined and it was found that they were resistance to florfenicol, erythromycin, oxytetracycline and trimethoprim-sulphamethoxazole which have been licensed for fish health in Turkey. It is considered that findings obtained will form a basis to develop diagnosis kit and/or vaccination for Y. ruckeri.