Research Information System
Eskişehir Technical University Journal of Science and and Technology A- Applied Sciences and Engineering, vol.21, no.1, pp.199-204, 2020 (Peer-Reviewed Journal)
Hyoscyamus reticulatus is a member of the family Solanaceae and is represented by six species in Turkey. Hyoscyamus species contain important tropane alkaloids, which have antispasmodic, anticholinergic, analgesic, and sedative effects. In this study, we aimed to determinate the contents of Hyoscyamus reticulatus seeds by X-ray fluorescence (XRF) spectrometry and investigate the antibacterial activity of the ethanol extract of H. reticulatus seeds. Energy-dispersive XRF spectrometry is one of the important approaches used to determine the elemental and chemical composition of all types of liquids and solids, allowing quick and accurate qualitative and quantitative analysis of elements. In this study, contents of H. reticulatus seeds were determined by XRF method as follows: 1.98% K2O (1.65 K%), 1.22% P2O5 (0.53 P%), 0.80% SO3 (0.32% S), 0.49% CaO (0.35 Ca%), 0.26% Fe2O3 (0.18% Fe), 0.13% Cl, 0.11% MgO (0.07% Mg), 0.10% ZnO (0.08% Zn), 0.06% MoO3 (0.04% Mo), 0.05% SiO2 (0.02% Si), 0.02% Cu, and 0.02% MnO (0.01% Mn), while NiO (67 ppm) and Al2O3 (94 ppm) were determined at the ppm level. H. reticulatus seeds were treated by extraction for antibacterial activity tests and Escherichia coli, Pseudomonas aeruginosa, Pasteurella multocida, Yersinia enterocolitica, Klebsiella pneumoniae, Staphylococcus aureus, and Salmonella enteridis bacteria were used. The extract affected no bacteria except for Pasteurella multocida (10 mm).