Research Information System
Van Veterinary Journal, vol.35, no.1, pp.32-37, 2024 (Peer-Reviewed Journal)
Cryoprotectants are used to protect cells in freezing; however, the optimal type and concentration of cryoprotectants vary depending on the cell type, and identifying the most effective combination can be challenging. This study compared the cryoprotection efficacy of dimethyl sulfoxide (DMSO), glycerol, and methanol on HeLa cell line. Material and Methods: Cell suspensions were frozen with a freezing media composed of cryoprotectant + fetal bovine serum + medium at a ratio of 5:10:85 (v: v: v) and stored under the following conditions: 3 months (-20°C), 1 month (-80°C), and 6 months (-80°C). Cell viability and recovery rates were analyzed immediately post-thaw and after 48 h using the trypan blue dye exclusion assay. Results: In 3 months (-20°C), viability and recovery rates were higher in the methanol group. Glycerol showed better performance in 1 month (-80°C). DMSO was the most efficient in 6 months (-80°C). Methanol failed at -80°C storage temperature. Conclusion: This study demonstrates the effect of these cryoprotectants in HeLa cells on cell viability and cell recovery rates immediately after thawing and after 48 hours of cultivation.