Akademik Veri Yönetim Sistemi
10. Mediterranean Symposium on Medicinal and Aromatic Plants, İstanbul, Türkiye, 25 - 27 Nisan 2024, ss.295-302, (Tam Metin Bildiri)
The purpose of the investigation was to determine the tyrosinase, elastase and cyclooxygenase enzyme inhibition activities and chemical constituents of above-ground of Tanacetum polycephalum subsp. argyrophyllum gathered from Van province of Turkey in June 2021. The plant extracts were prepared with nhexane, ethyl acetate and ethanol using the maceration method. The essential oil was extracted using the Clevenger apparatus. The chemical profile of the extracts was evaluated by LC-MS/MS, and the chemical profile of the essential oil was evaluated by GC and GC-MS. The inhibitory activity of the samples against tyrosinase was tested at concentrations ranging from 100 to 500 µg/ml, and the extracts showed higher inhibition activity than the essential oil. While the extracts had no inhibitory effect on elastase, the essential oil had a weak effect at 500 µg/ml. None of the samples had a significant inhibitory effect on cyclooxygenase enzymes at 100 µg/ml. The yield of essential oil was found to be 1%. Thirty-five compounds were detected in the essential oil. Camphor (29.68%), di-tert-butyl(methylamino)borane (24.25%), chrysanthenyl acetate (17.71%), bicyclo[3.1.1]hept-2-en-4-ol, 2,6,6-trimethyl acetate (16.64%) and cyclopentan-1-al, 4-isopropylidene-2- methyl (7.22%) were defined as main constituent of the essential oil. Cyranoside, quinic acid, chlorogenic acid, cosmosiin, luteolin, acacetin and protocatechuic acid were the major compounds of the extracts.