Micromonospora deserti sp. nov., isolated from the Karakum Desert

Saygın H., AY H., Guven K., ÇETİN D., ŞAHİN N.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol.70, no.1, pp.282-291, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 70 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.1099/ijsem.0.003752
  • Journal Name: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.282-291
  • Keywords: Micromonospora, phylogenomics, Karakum Desert, polyphasic taxonomy, RIBOSOMAL-RNA GENE, INTRAGENERIC RELATIONSHIPS, SEQUENCE, DATABASE, NOCARDIA, MEMBER
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

An isolate, 13K206(T), with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert. A polyphasic approach was adopted to determine taxonomic affiliation of the strain. The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A(2)pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids. The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1(T), Micromonospora nigra DSM 43818(T) and Micromonospora yasonensis DS3186(T) with sequence similarities of 98.6, 98.5 and 98.4 %, respectively. Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp. nov. is proposed. The type strain is 13K206(T) (=JCM 32583(T)=DSM 107532(T)). The DNA G+C content of the type strain is 72.4 mol%.