Streptomyces scabichelini sp. nov., isolated from soil

Gencbay T., Saygın H., Guven K., Topkara A. R., Sarıcaoğlu S., ŞAHİN N., ...More

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol.71, no.2, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 71 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.1099/ijsem.0.004639
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Keywords: Actinobacteria, MLSA, phylogenomic, Streptomyces scabichelini sp. nov., soil, 16S rRNA gene, HETEROTYPIC SYNONYMS, GENOME, CLASSIFICATION, SEQUENCES, TAXONOMY, DATABASE, TREES
  • Ondokuz Mayıs University Affiliated: Yes


A novel actinomycete, designated strain HC44(T), was isolated from a soil sample collected from Hacibektas, Turkey, and characterized using a polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members of the genus Streptomyces. Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that HC44(T) clustered with members of the genus Streptomyces and the highest 16S rRNA gene sequence similarity values were obtained with Streptomyces vastus NBRC 13094(T) (97.6%) and Streptomyces kalpinensis TRM 46509(T) (96.9%). Multi-locus sequence analysis (MLSA) based on five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed that the MLSA evolutionary distance value was 0.043 between strain HC44(T) and S. vastus NBRC 13094(T). Whole-cell hydrolysates contained LL-diaminopimelic acid, glucose, mannose and ribose. The predominant menaquinones were MK-9(H-6) and MK-9(H-8). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The DNA G+C content of the draft genome sequence, consisting of 11.2 Mbp, was 69.8 mol%. On the basis of polyphasic taxonomic evidence, strain HC44(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces scabichelini sp. nov. is proposed. The type strain is HC44(T) (=DSM 106874(T)=KCTC 39872(T)).