Interleukin-1Ra rs2234663 and Interleukin-4 rs79071878 Polymorphisms in Familial Mediterranean Fever

Nursal A. F., Tekcan A., Kaya S. U., Sezer O., YİĞİT S.

Gene, vol.582, no.2, pp.173-177, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 582 Issue: 2
  • Publication Date: 2016
  • Doi Number: 10.1016/j.gene.2016.02.008
  • Journal Name: Gene
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.173-177
  • Keywords: Familial Mediterranean Fever, IL-1Ra, IL-4, Polymorphism
  • Ondokuz Mayıs University Affiliated: No


Objective: Familial Mediterranean Fever (FMF) is an autosomal recessively inherited auto inflammatory disorder. MEFV gene, causing FMF, encodes pyrin that is associated with the interleukin-1 (IL-1) related inflammation cascade. The aim of this study was to investigate the relationship of interleukin-1 receptor antagonist (IL-1Ra) and interleukin-4 (IL-4) polymorphisms with the risk of FMF in the Turkish population. Methods: This study included 160 patients with FMF (74 men, 86 women) and 120 healthy controls (50 men, 70 women), respectively. Genotyping of IL-1Ra rs2234663 polymorphism was evaluated by gel electrophoresis after polymerase chain reaction (PCR). The IL-4 rs79071878 polymorphism was determined by PCR-based restriction fragment length polymorphism (PCR-RFLP) analysis. The results of analyses were evaluated for statistical significance. Results: There was no significant difference in IL-1Ra genotype and allele distributions between FMF and the control groups (p > 0.05). However, a significant association was observed between FMF patients and control groups according to IL-4 genotype distribution (p = 0.016), but no association was found in the allelic frequency of IL-4 between FMF patients and the controls (p > 0.05, OR: 1.131, CI 95%: 0.71-1.81). Conclusions: The IL-4 rs79071878 polymorphism, was associated whereas the IL-1Ra rs2234663 polymorphism was not associated with FMF risk in the Turkish population. Larger studies with different ethnicities are needed to determine the impact of IL-1Ra and IL-4 polymorphism on the risk of developing FMF.