The objective of this study was to determine the effects of substrates prepared by the mixtures of sawdust and wood-chip of oak with tea manufacture waste (TW) at the rate of 10, 15, 20 and 25% on the yield and some morphological characters of Ganoderma lucidum mushroom. Ganoderma lucidum was obtained from Company of Agromycel in Denizli. In the study, pH, moisture, N and C contents, and C:N ratios of substrates, and also days to first fruiting, yield and some morphological characters were determined. Substrate mixtures of sawdust (OS) and wood-chip (OWC) of oak at the rate of 80% with 18% wheat brain (WB), 1% sucrose and 1% CaCO3 were used as their controls. The highest mushroom yield was obtained from 90 OWC:10 TW (73.07 g/kg substrate) and 75OS:25TW (21.21 g/kg substrate). It was determined that mushroom yields of the substrates, except for the mixtures of 80:20TW, 85:15TW and 90:10TW prepared by oak sawdust with tea waste, were higher than that in control substrate of 80:18WB.