Blockade of p-selectin reduces neutrophil infiltration into the murine testis after ischemia-reperfusion-injury

Celebi M., Paul A. G. A.

DEUTSCHE TIERARZTLICHE WOCHENSCHRIFT, vol.115, no.12, pp.457-460, 2008 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 115 Issue: 12
  • Publication Date: 2008
  • Doi Number: 10.2376/0341-6593-115-457
  • Journal Name: DEUTSCHE TIERARZTLICHE WOCHENSCHRIFT
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.457-460
  • Keywords: antibodies, neutrophils, selectin, testis, torsion, CELL-ADHESION MOLECULES, EXPRESSION, BLOCKING, PROTECTS
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

Germ cell specific apoptosis after ischemia-reperfusion (I/R) induced testicular injury is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E-selectins play an important role in this recruitment. The purpose of this study was to inhibit neutrophil recruitment in I/R induced testicular injury by using a function-blocking monoclonal anti-mouse P-selectin antibody, Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion). Ten minutes after the onset of reperfusion, mice received either 100 mu g of a function-blocking monoclonal P-selectin antibody (FBMAB group) or isotype-matched control antibody (IMCA group). Separate groups of mice underwent sham-operation (SO group) or received 500 ng of TNF alpha (IF group) to induce inflammation, Mice were sacrificed 24 h after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry.