The assessment of the protein profiles and oxidant/antioxidat status in conjunctival Brucella melitensis Rev1 vaccinated sheep

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Etlik Veteriner Mikrobiyoloji Dergisi, vol.32, no.2, pp.101-106, 2021 (Peer-Reviewed Journal) identifier


Brucellosis is a zoonotic and economically significant animal disease worldwide. The most frequently used vaccine to avoid brucellosis in small ruminants is the Rev1 conjunctival Brucella melitensis vaccine. The aim of this study was to investigate the effects of B.melitensis Revl conjunctival vaccine on total protein, albumin, globulin levels, protein profiles and oxidant/antioxidant status in sheep. Ten sheep were used as animal material for this purpose. The bloods taken before vaccination were used as negative control. The sera obtained one month after administration of single dose B.melitensis vaccine were used as experimental materials. The spectrophotometric method estimated total protein, albumin, globulin levels, total antioxidant capacity (TAS), and total oxidant capacity (TOS). Protein profile was determined by sodium-dodecyl-sulphate and native-polyacrylamide gel electrophoresis methods. It was determined that total protein and globulin levels increased slightly in sero positive sheep (P>0.05). There was no difference for protein profiles in both electrophoresis methods. The band densities of albumin decreased but gamma globulin increased slightly after vaccination. TAS levels decreased significantly (P<0.05), but TOS levels increased slightly (P>0.05). In conclusion, Rev-1 conjunctival Brucella vaccine was thought to be safe to use to prevent Brucellosis, and the addition of antioxidant after vaccination can reduce oxidative stress.