The objective of this study was to evaluate the effect of sodium hypochlorite and chlorhexidine solutions and their inactivating agents on the push-out bond strength of mineral trioxide aggregate (MTA). One hundred fifty root slices were prepared and their root canal spaces were enlarged using diamond burs. MTA was prepared and compacted to root canal spaces. The samples were randomly separated to 4 groups that would be immersed into the selected solutions (5.25% sodium hypochlorite (NaOCl), 5.25% NaOCl and sodium thiosulfate, 2% chlorhexidine (CHX), 2% CHX and a mixture of Tween 80 and 0.3% L-alfa-lecithin) and a control group (n = 30). Push-out bond strength of each specimen was tested with universal testing machine. The data was analyzed by one-way analysis of variance and Tukey tests. There were no significant differences between the push-out bond strength values of the irrigation groups and the control group. A significant difference was found between the push-out bond strength values of NaOCl-Sodium thiosulfate and CHX-L-alpha-lecithin groups. Contact with NaOCl and its neutralizing agent with MTA after 10 minutes of setting period increased the push-out bond strength of MTA. On the other hand, any contact with CHX alone or with subsequent application using L-alfa-lecithin should be avoided since that might decrease the push-out bond strength of freshly mixed MTA.