The therapeutic effects of anti-oxidant and anti-inflammatory drug quercetin on aspiration-induced lung injury in rats


Yilmaz M. Z., Guzel A., Çağlar Torun A., Okuyucu A., Salis O., Karli R., ...Daha Fazla

JOURNAL OF MOLECULAR HISTOLOGY, cilt.45, sa.2, ss.195-203, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 2
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1007/s10735-013-9542-3
  • Dergi Adı: JOURNAL OF MOLECULAR HISTOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.195-203
  • Anahtar Kelimeler: Lung injury, Aspiration pneumonitis, Quercetin, iNOS, CC-16, GASTROINTESTINAL DECONTAMINATION AGENTS, SECRETORY PROTEIN CC16, OXIDATIVE STRESS, ACTIVATED-CHARCOAL, FREE-RADICALS, NITRIC-OXIDE, BIOMARKER, CURCUMIN, DISEASE, CELLS
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Aspiration pneumonitis refers to acute chemical lung injury caused by aspiration of sterile gastric contents. The aim of this study was to evaluate the role of quercetin (QC) in acid aspiration-induced lung injury in rats. Twenty-eight female Sprague-Dawley rats were used and divided into the following groups (n = 7): sham (aspirated normal saline, S), hydrochloric acid (aspirated HCl), S plus treatment with QC (S + QC), and HCl plus treatment with QC (HCl + QC). After aspiration, the treatment groups received QC 60 mg/kg/day intraperitoneally once a day for 7 days. As a result of acid aspiration, an increase was observed in the levels of serum clara cell protein-16 (CC-16) and advanced oxidation protein products, whereas there was a decrease in serum thiobarbituric acid-reactive substances, superoxide dismutase (SOD), and catalase levels. There was a significant decrease in peribronchial inflammatory cell infiltration, alveolar septal infiltration, alveolar edema, and alveolar exudate scores, except in the alveolar histiocytes in the HCl + QC group. The expression of nitric oxide synthase, which increased after aspiration in the HCl group, showed a statistically significant decrease after the QC treatment. After the treatment with QC, an increase in the serum SOD level was observed, whereas a significant decrease was determined in the serum CC-16 level relative to that of the aspiration group (HCl). The antioxidant QC is effective in the treatment of lung injury following acid aspiration and can be used as a serum CC-16 biomarker in predicting the severity of oxidative lung injury.