A comparative study of parthenogenetic activation and in vitro fertilization of in vitro matured bovine oocytes

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Çevik M., Tas A., Akkoc T., Bagis H., Arat S.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, vol.33, no.5, pp.393-399, 2009 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 5
  • Publication Date: 2009
  • Doi Number: 10.3906/vet-0803-5
  • Journal Name: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.393-399
  • Keywords: Maturation, in vitro fertilization, parthenogenetic oocyte activation, embryo culture, NUCLEAR TRANSFER EMBRYOS, CHEMICAL ACTIVATION, ETHANOL, SPERM, CYCLOHEXIMIDE, PLOIDY, CATTLE, SERUM
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

The objective of this study was to determine the effects of 3 chemical agents used sequentially and electrical stimuli on parthenogenetic activation of in vitro matured bovine oocytes and comparison with a standard IVF protocol for embryo developmental rates. For this purpose, oocytes were matured in tissue culture medium-199 (TCM-199) at 39 C and 5% of CO(2) in humidified air. For IVF, matured oocytes were fertilized in Modified Tyrode-Lactate Medium. In the parthenogenetic activation process, direct current (DC) was pulsed (133 V/500 mu m) for 25 mu s and oocytes were sequentially activated with calcium ionophore (CaI) for 10 min, cycloheximide (CHX) + cytochalasin D for 1 h, and CHX for 5 h. After that, all embryos (both IVF and parthenogenetic) were cultured in G1.3/G2.3 media containing 6% CO(2), 5% O(2), and 89% N(2) in humidified air at 39 degrees C.