The Effect of Bacillus megaterium var. Phosphaticum Applied With İnorganic Phosphate Sources On Biological Properties and İnorganic Phosphorus Fractions of Calcareous Soil
Journal of Soil Science and Plant Nutrition, cilt.26, sa.3, ss.9374-9393, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 26 Sayı: 3
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s42729-026-03475-3
- Dergi Adı: Journal of Soil Science and Plant Nutrition
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Natural Science Collection (ProQuest), Biological Science Database (ProQuest)
- Sayfa Sayıları: ss.9374-9393
- Anahtar Kelimeler: Phosphatase activity, Phosphate-solubilizing bacteria, Phosphorus availability, Rock phosphate, Triple superphosphate
- Ondokuz Mayıs Üniversitesi Adresli: Evet
Özet
This study investigates the impact of Bacillus megaterium var. phosphaticum, in conjunction with inorganic phosphate sources, on the biological properties and inorganic phosphorus (P) fractions of calcareous soil. This study investigated the effects of indigenous (RK 01) and reference (DSM 3228) strains of Bacillus megaterium var. phosphaticum, applied with different inorganic P sources (Rock Phosphate [RP] and Triple Superphosphate [TSP]), on soil inorganic P fractions and biological properties over a 120-day incubation period. The results showed that bacterial inoculation significantly enhanced P availability and microbial activity across all treatments. The indigenous strain RK 01 demonstrated superior performance in mobilizing stable P pools, particularly by limiting the percentage accumulation of the calcium-bound P (Ca-P) fraction compared to the non-inoculated controls. A remarkable synergistic effect was observed between TSP and bacterial strains, where TSP_A (TSP + RK 01) achieved the highest levels of Olsen-P and soluble and loosely bound P (SLB-P). Notably, soil phosphatase activity (PA) reached its peak increase in the TSP_A treatment at day 90, highlighting the potent enzymatic potential of the indigenous strain. Furthermore, a significant decrease in the reductant soluble P (RS-P) fraction suggested a net transformation of recalcitrant P into more mobile forms driven by increased microbial biomass carbon (MBC) and basal respiration (BSR) rates. These findings suggest that the use of indigenous Bacillus megaterium strains, especially when integrated with inorganic P fertilizers, provides a sustainable strategy to improve P recovery and soil biological health in lime-rich agricultural environments.