Phylogenetic analysis of Rhizoctonia solani AG-4 isolates from common beans in Black Sea coastal region, Turkey, based on ITS-5.8S rDNA

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ÇEBİ KILIÇOĞLU M., Özkoç İ.

TURKISH JOURNAL OF BIOLOGY, vol.37, no.1, pp.18-24, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 1
  • Publication Date: 2013
  • Doi Number: 10.3906/biy-1202-17
  • Journal Name: TURKISH JOURNAL OF BIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.18-24
  • Keywords: rDNA-ITS phylogeny, Rhizoctonia solani, AG-4, Phaseolus vulgaris, common bean, ANASTOMOSIS GROUPS, WEB BLIGHT, PHASEOLUS-VULGARIS, HYPOCOTYL ROT, RIBOSOMAL DNA, COSTA-RICA, PATHOGENICITY, DIFFERENTIATION, IDENTIFICATION, THANATEPHORUS
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

In this study, 114 Rhizoctonia solani isolates were obtained from diseased common bean (Phaseolus vulgaris L.) plants from the Black Sea coastal region of Turkey. Genetic variation was determined among R. solani anastomosis group 4 (AG-4) subgroups (AG-4 HG-I and AG-4 HG-II). ITS-5.8 rDNA sequences of these isolates were aligned with other known R. solani sequences from GenBank, and distance and parsimony analysis were used to determine phylogenetic relationships. The R. solani AG-4 isolates were placed in 2 main lineages, corresponding to AG-4 HG-I and AG-4 HG-II. Based on phylogenetic analysis, genetic variations were shown in the AG-4 subgroups (especially AG-4 HG-I). This study represents the first reported use of rDNA-ITS sequence data to examine AG-4 HG-I and HG-II in P. vulgaris from Turkey. The results also suggest that the Giresun isolates are closely related to isolates obtained from Italy