Objective: Many drug discovery have used nature as an inspiration for the design of naturel products like compound classes. From ancient times edible mushrooms have been used both as food and medicine. People living in Turkey widely consume Suillus luteus (L.) Roussel wild edible mushrooms In this study, we were investigated antioxidant, antimicrobial and cytotoxic activities of various extracts of S.luteus. Material and Method: Antioxidant activity of S.luteus was detected method by DPHH free radical scavenging and ferrous ion chelating ability. In addition, the concent of the components with antioxidant properties, such as total phenols,β-caratone and lycopene were determined by spectrophotometric methods. The antimicrobial potential was demonstrated with a agar well diffusion method on 14 microorganisms. Finally, the cytotoxic effect of methanolic extract of S. luteus on MCF-7 cancer cell lines were evaluated by using MTT method. Result and Discussion: The results indicated that S.luteus methanolic and ethanolic extracts have more abundant phenols (153, 49.33 mg GAE/g extract, respectively).In addition β-caratone and lycopene content detected. (from 0.120 to 0.606μg/mL).S.luteus extracts had more potent free radical scavenging activity than standard antioxidants BHT. (Methanol extract (IC50: 63.72μg/mL) > Ethanol extract (IC50: 80.72 μg/mL) > BHT (IC50: 96.47μg/mL). In addition, methanol extracts possessed higher ferrous ion chelating ability than ethanol extracts(2.72, 3.45 μg/mL, respectively) .Generally, the tested mushroom extracts had relatively low antimicrobial activity against the tested microorganisms (9 and 10 mm zone diameter). Also, S.luteus methanolic extract was found to kill all cancer cells at a concentration of 1mg/mL. These results showed that S.luteus, especially methanol extracts, have potential medical.