Purpose This study was conducted to investigate the fracture strength of reattached tooth fragments after different cavity disinfection protocols. Materials and Methods Incisal edges of 144 bovine incisors were sectioned and then randomly divided into 4 different groups as follows: Group C: no disinfectant; Group NaOCl: 2.5% sodium hypochlorite solution; Group NaOCl+Asc: 2.5% sodium hypochlorite followed by 10% ascorbic acid solution; Group CHX: 2% chlorhexidine solution. Teeth were further divided into 3 subgroups according to universal adhesive (G-Premio Bond, Scotchbond Universal, Prime and Bond Universal). Fracture resistance was evaluated using a universal testing machine. Data was analyzed using 2-way ANOVA with Bonferroni tests. Results Cavity disinfectant had a statistically significant effect on bond strength (p<0.05), with the highest bond strength detected in the NaOCl+Asc Group (148.22 +/- 51.64) and the lowest in the NaOCl Group (112.84 +/- 43.12). Scotchbond Universal exhibited the highest bond strength values (163.59 +/- 49.94); however, there were no significant differences between the other adhesive systems (p> 0.05). . Conclusion Application of ascorbic acid following sodium hypochlorite treatment can improve dentin bond strength.