Research Information System
TAXONOMY, vol.5, no.5, pp.1-13, 2025 (ESCI)
Actinomycetes are known to produce a vast array of bioactive secondary metabolites
with potential therapeutic applications, including antimicrobials, anticancer agents,
and enzyme inhibitors. Among these, members of the genus Nonomuraea have received
much attention due to their broad ecological importance in nutrient cycling in soil and
their ability to produce new bioactive compounds. A novel actinomycetes, designated
strain M3C6T, was isolated from soil samples collected on Marmara Island, located in
the Istanbul province, aiming to explore the microbial diversity of unexplored habitats,
and characterized using a polyphasic approach. The isolate showed chemotaxonomic
and morphological features consistent with members of the genus Nonomuraea. The 16S
rRNA gene sequence analysis revealed that strain M3C6T shared the highest similarity, at
98.7% sequence identity, to Nonomuraea basaltis 160415T and Nonomuraea turkmeniaca DSM
43926T. However, the ANI and dDDH values between strain M3C6T and these reference
strains were fairly low, ranging from 84.0 to 84.6% and 31.8 to 33.7%, respectively, below the
generally accepted cutoffs for ANI and DDH that delineate different prokaryotic species.
Genomic analysis of strain M3C6T showed that it had a genome size of 10.38 Mbp and
a DNA G+C content of 69.5 mol%. Based on these chemotaxonomic, phenotypic, and
genomic data, strain M3C6T is classified as a novel species within the genus Nonomuraea,
for which the name Nonomuraea marmarensis sp. nov. is proposed. The type strain is M3C6T
(= KCTC 49983T = CGMCC 4.8035T). Genomic analyses confirmed the high potential of
M3C6T to produce specialized secondary metabolites.