Aim This study aimed to test the validity and reliability of the Turkish version of the Nursing Teamwork Survey, which was developed to measure the concept of nursing teamwork. Method Performed as a methodological study with a cross-sectional design, the study included 486 bedside nurses in three different types of hospitals in Istanbul. The data were collected using the Nursing Teamwork Survey between October and December 2018. The scale includes five subscales and 33 items. The data were analysed using SPSS 22.0 and LISREL 8.7. Results Nurses' mean age was 29.82 (SD= 7.33). The content validity index of the scale was 0.94. Confirmatory factor analysis conducted on the original form showed that all items fell within the subscales of the original scale and that their factor loads were between 0.48 and 0.91. Following factor analysis, the factor structure of the original scale was valid; however, suggested modifications were applied to improve the results. The obtained goodness-of-fit indices were close to the excellent range according to literature. Cronbach's alpha was 0.95 for the scale. Conclusion The scale is a valid and reliable tool for measuring teamwork among nurses.