Akademik Veri Yönetim Sistemi
FOOD ANALYTICAL METHODS, cilt.18, sa.8, ss.1621-1636, 2025 (SCI-Expanded)
This study sought to maximize the extraction of Glebionis coronaria's total phenolic content (TPC) and total flavonoid content (TFC), determining the best conditions for separating these beneficial substances. Liquid-liquid partitioning was carried out after extraction using solvents of different polarities, such as n-butanol, ethyl acetate, and chloroform. While antioxidant activity was evaluated using DPPH, ABTS, reducing power, phenanthroline, and silver nanoparticle assays, TPC and TFC were measured using the Folin-Ciocalteu and AlCl3 techniques, respectively. Cytotoxic effects were evaluated against two cancer cell lines (CAPAN-1 and dld-1) as well as a healthy cell line (L929), and the ethanolic extract's substance composition was examined using LC-ESI-MS/MS. The optimal TPC and TFC extraction parameters were found to be X1 (48 h), X2 (70% ethanol), and X3 (30 mL/g). The n-butanol fraction showed significant antioxidant activity (IC50: 13.89 mu g/mL for DPPH, 29.18 mu g/mL for ABTS) and had the highest TPC (325.33 mg GAE/g dw) and TFC (112.5 mg QE/g dw). Additionally, it demonstrated a considerable ability to chelate metals. Twelve bioactive compounds were identified in the ethanolic extract, with chlorogenic acid being the main component (422.47 mu g/g). Cytotoxicity testing revealed significant effects against CAPAN-1 cells, reducing viability by 38.53% at 1 mg/mL. An ADME model provided insights into the pharmacokinetics and bioavailability of the identified compounds, highlighting their therapeutic applications.