The role of iNOS inhibitors on lung injury induced by gastrointestinal decontamination agents aspiration

Guzel A., Guzel A., Gunaydin M., Alacam H., Salis O., Paksu M. S., ...More

JOURNAL OF MOLECULAR HISTOLOGY, vol.43, no.3, pp.351-360, 2012 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 43 Issue: 3
  • Publication Date: 2012
  • Doi Number: 10.1007/s10735-012-9397-z
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.351-360
  • Keywords: Activated charcoal, Aspiration, Pulmonary injury, Polyethylene glycol, Surfactant protein-D, S-methylisothiourea, SURFACTANT PROTEIN-A, GLYCOL-ELECTROLYTE SOLUTION, ACUTE PULMONARY-EDEMA, ACTIVATED-CHARCOAL, COMPLICATIONS, BRONCHIOLITIS, PNEUMONIA, MODEL, RISK
  • Ondokuz Mayıs University Affiliated: Yes


Aspiration is a devastating complication during decontamination procedure in poisoning patients. We have investigated whether S-methylisothiourea protects different pulmonary aspiration gastrointestinal decontamination agent-induced lung injury in rats. Forty-two male Sprague-Dawley rats were assigned to one of six groups (n = 7): normal saline, activated charcoal, polyethylene glycol, normal saline + S-methylisothiourea treated activated charcoal + S-methylisothiourea treated and polyethylene glycol + S-methylisothiourea treated. Normal saline, activated aharcoal and polyethylene glycol were instilled into the lungs. The rats received S-methylisothiourea i.p twice daily for 7 days. Serum surfactant protein D, oxidative stress products and inducible nitric oxide synthase expression in the lung were investigated. The aspiration of activated charcoal significantly increased all histopathological scores (P < 0.01). Only peribronchial inflammatory cell infiltration, alveolar edema, and alveolar histiocytes were increased in the polyethylene glycol groups as compared to the normal saline group (P < 0.05). Pulmonary aspiration increased serum malondialdehyde (P < 0.001), and surfactant protein D (P < 0.05) levels and decreased serum superoxide dismutase levels (P < 0.05). S-methylisothiourea treatment decreased all histopathological scores in the activated charcoal treated S-methylisothiourea group (P < 0.01) and only decreased alveolar edema and alveolar histiocytes in the polyethylene glycol-treated S-methylisothiourea group (P < 0.05). S-methylisothiourea treatment reduced elevated oxidative factors, inducible nitric oxide synthase activity and serum surfactant protein D levels. Our findings showed that S-methylisothiourea may be a protective drug against Activated Charcoal and Polyethylene Glycol-induced lung injury.