Evaluation of immunoblotting test results in patients with positive antinuclear antibodies Antinükleer antikorların pozitif saptandığı hastalarda immunoblotting test sonuçlarının değerlendirilmesi


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Vural D. G., Çayci Y. T., Bıyık İ., BİLGİN K., Birinci A.

Turk Hijyen ve Deneysel Biyoloji Dergisi, vol.78, no.4, pp.443-450, 2021 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 78 Issue: 4
  • Publication Date: 2021
  • Doi Number: 10.5505/turkhijyen.2021.24482
  • Journal Name: Turk Hijyen ve Deneysel Biyoloji Dergisi
  • Journal Indexes: Scopus, Academic Search Premier, CAB Abstracts, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.443-450
  • Keywords: Ana (antinuclearantibody), Extractable nuclear antigen (ena), Indirect immunofluorescence antibody (iifa)
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

Objective: Autoimmune diseases occur as a result of the immune response to self antigens and tissuesof the organism and the detection of autoantibodiesis very important in the diagnosis of these disorders.Antinuclear antibodies (ANA) autoantibodies generatedagainst to nuclear/ cytoplasmic components of the cellare important diagnostic criteria for connective tissuediseases. Currently, a number of methods are availablefor the detection of ANA. The gold standard method forthe detection of ANA is Indirect ImmunofluorescenceAntibody (IIFA) assay using Hep2 (human laryngealepidermoid carcinoma). When positive results areobserved, Extractable Nuclear Antigen( ENA) tests followas a means of confirming the diagnosis. Identification ofthe specific extractable nuclear antigens is warrantedbecause this may further differentiate between thedistinct types of autoimmune connective tissue diseases.In this study, were analyzed retrospectively that ENAtest results in patients with positive ANA IIFA test.Methods: Antinuclear antibodies were tested for atotal of 3000 patients admitted to various clinics of OndokuzMayıs University Medical Faculty. Each serum samplewas studied at 1: 100 dilution in accordance with the manufacturer’s recommendations (Euroimmun AG, Lübeck,Germany) and staining pattern with fluorescence intensitywas evaluated with immunofluorescence microscope. ENAwas investigated by immunoblotting method (EuroimmunAG, Lübeck, Germany) in 640 ANA positive sera.Results: Distribution of the patients included in thestudy, 2192 (73.07%) were female and 808 (26.93%) weremale. 640 samples detected as ANA positive. When we lookat the distribution of ANA patterns in positive samples;granular 173 (27.03%), granular and cytoplasmic granular98 (15.31%), homogenous and granular 67 (10.47%) werethe most common. When the ENA profiles of the ANApositivesamples were examined, 557 (83.7%) were foundto be positive and 83 (12.97%) were negative. According toour study, SSA (26.88%), SSB (17.81%), Sm / RNP (17.66%)were the first three places in the ENA positivite.Conclusion: Our ANA positivity rate was foundto be compatible with the literature. The patterndistribution is similar to the data of our region. Afterthe first screening with IIFA, looking for differentantigens with the immunoblot test; not only it is a costeffective approach;but also facilitate the diagnosis ofautoimmune diseases