A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain K220(T), was isolated from soil collected from Cape Andreas (Zafer Burnu), Northern Cyprus, and subjected to a polyphasic taxonomic approach. The organism was shown to have phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Saccharopolyspora. 16S rRNA gene sequence analysis of strain K220(T) showed that it is closely related to the type strains of Saccharopolyspora maritima 3SS5-12( T), Saccharopolyspora kobensis JCM 9109( T) and Saccharopolyspora hirsuta ATCC 27875( T) with 97.6, 97.5 and 97.0% sequence similarity, respectively. In silico DNA-DNA hybridization and average nucleotide identity values between strain K220(T) and type strains of the genus Saccharopolyspora with publicly available genomes were 22.1-31.2% and 76.0-83.16%, respectively. The DNA G + C content of strain K220(T) was 68.3 mol%. The genome of strain K220(T) has genes associated with 24 biosynthetic gene clusters. The strain contained MK-9(H-4) and iso-C-16: 0 as the predominant respiratory quinone and fatty acid, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine. Based on evidence collected from the genotypic, phenotypic and phylogenetic analyses, strain K220(T) is considered to represent a novel species in the genus Saccharopolyspora, for which the name Saccharopolyspora soli sp. nov. is proposed. The type strain is K220(T) (= JCM 33912(T) = KCTC 49395(T)).