Production of androgenetic Nile tilapia, Oreochromis niloticus L.: Optimization of heat shock duration and application time to induce diploidy

Karayücel S., Karayücel I., Penman D., McAndrew B.

Israeli Journal of Aquaculture - Bamidgeh, vol.54, no.4, pp.145-156, 2002 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 54 Issue: 4
  • Publication Date: 2002
  • Journal Name: Israeli Journal of Aquaculture - Bamidgeh
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.145-156
  • Keywords: Androgenesis, Diploidy, Tilapia
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

Diploid androgenetic Nile tilapia, Oreochromis niloticus, were produced using 5 min UV irradiation at 150 μW/cm and heat shock at 42.5°C for 3:30 min applied 25 min after fertilization. The survival rates to morula, pigmentation, hatching and yolk sac resorption stages were 42.84±4.73%, 10.69±2.24%, 2.03±0.60% and 0.07±0.07% (relative to controls), respectively. A significant female, but not male, effect on the survival was confirmed. Multilocus DNA fingerprinting produced by hypervariable 33.15 DNA probe verified the all-paternal inheritance in the androgens. The sex ratio of the androgenetic tilapia was not significantly different from the expected ratio of 1:0 (male:female), indicating that O. niloticus has a monofactorial sex-determining mechanism with female homogamety and male heterogamety. This study confirmed that the YY genotype is viable and fertile in O. niloticus.