Trichuris trichiura were collected from rhesus macaque (Macaca mulatta) and Hamadryas baboon (Papio hamadryas) in Samsun Zoo, Turkey. DNA was isolated from individual worm in baboon for molecular characterization. The ITS region was amplified and sequencing in both directions using NC5-NC2 primers. Pairwise comparison between ITS region of the T. trichiura isolates from Turkey (KC877992) and other isolates China (AM992981, AM992985, AM992998), Czech Republic (JF690940, JF690941, JF690950), Netherlands (JF690948) and South Africa (GQ301551, GQ301553) showed differences ranging from 0.1% to 1.2%. With the present study, T. trichiura from Turkey were characterized for the first time by sequencing of the ITS.