Isolation and culture of adult mouse vestibular nucleus neurons


Him A., Altuntas S., ÖZTÜRK G., ERDOĞAN E., Cengiz N.

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.47, sa.6, ss.1903-1911, 2017 (SCI-Expanded, Scopus, TRDizin) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 47 Sayı: 6
  • Basım Tarihi: 2017
  • Doi Numarası: 10.3906/sag-1706-158
  • Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.1903-1911
  • Anahtar Kelimeler: Vestibular nucleus, primary culture, regeneration, mouse, whole-cell recording, RAT HIPPOCAMPAL-NEURONS, GANGLION NEURONS, RESPONSIVENESS
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Background/aim: Isolated cell cultures are widely used to study neuronal properties due to their advantages. Although embryonic animals are preferred for culturing, their morphological or electrophysiological properties may not reflect adult neurons, which may be important in neurodegenerative diseases. This paper aims to develop a method for preparing isolated cell cultures of medial vestibular nucleus (MVN) from adult mice and describe its morphological and electrophysiological properties.