Isolation and culture of adult mouse vestibular nucleus neurons

Him A., Altuntas S., ÖZTÜRK G., ERDOĞAN E., Cengiz N.

TURKISH JOURNAL OF MEDICAL SCIENCES, vol.47, no.6, pp.1903-1911, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 6
  • Publication Date: 2017
  • Doi Number: 10.3906/sag-1706-158
  • Journal Name: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.1903-1911
  • Keywords: Vestibular nucleus, primary culture, regeneration, mouse, whole-cell recording, RAT HIPPOCAMPAL-NEURONS, GANGLION NEURONS, RESPONSIVENESS
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

Background/aim: Isolated cell cultures are widely used to study neuronal properties due to their advantages. Although embryonic animals are preferred for culturing, their morphological or electrophysiological properties may not reflect adult neurons, which may be important in neurodegenerative diseases. This paper aims to develop a method for preparing isolated cell cultures of medial vestibular nucleus (MVN) from adult mice and describe its morphological and electrophysiological properties.